4150 Lafayette Center Drive Suite 600 Chantilly Va 20151 Phone: (703)-222-8700

Instructions for Sampling and Shipping Adipose Tissues for Pesticide/PCB Screen Sampling

It is the policy of USDA to accept analyses of laboratories in the Accredited Laboratory Program as comparable to their own in-house laboratories, therefore USDA recommends that any sample received not conforming to these criteria be discarded without analysis

Sampling

IMPORTANT - SAMPLING IS THE STEP WHERE EXTERNAL CONTAMINATION OF THE TISSUES IS MOST LIKELY TO OCCUR. THEREFORE, PLEASE FOLLOW THE INSTRUCTIONS CAREFULLY.

   At random, select six (6) birds from the same flock. A flock is a group of birds from the same farm that may have been raised in different houses, but were fed the same feed, the same water, etc. Samples should be taken from each house according to the following table:

Number of Houses

1

2

3

4

5

6

7

8

9

10

Samples from each House

6

3

2

2+2+1+1

2+1+1+1+1

1

1

1

1

1

  1. Using the above table, if there was only one house for one flock then 6 birds would be sacrificed for testing. If there were 5 houses, for example, then 2 birds would be taken from one house and a single bird from the rest. If there are 6 or more houses for a flock, then 1 bird should be sacrificed from each house for testing. We also recommend that, to provide you with further assurance, there are six different samples; the spleens from each bird should be sent together with the abdominal fat as additional confirmation.

  2. Transfer the carcasses to a clean area where no contamination from dust, dirt, pesticides, herbicides or other chemicals can take place.

  3. Wash hands and knife or other equipment thoroughly before removing tissue.

  4. Remove one piece of fat tissue from the abdominal cavity of each bird.

  5. The sample of fat tissue from each bird should be approximately the diameter of a silver dollar and half inch thick or about the size that would fit on a tablespoon (approximately half ounce or 14 grams).

Packaging

IMPORTANT - PACKAGING IS THE MOST CRITICAL STEP TO AVOID LEAKAGE, PLEASE FOLLOW INSTRUCTIONS CAREFULLY.

  1. Each sample from each bird must be distinct and separate. Each piece should be placed in a 6 X 6 inch piece of aluminum foil, bagged and sealed in a plastic bag and labeled with the farm name and flock identifier. This individual wrapping insures that pieces of more than one bird do not intermingle and contaminate the others.

     

  2. Place each foil-wrapped sample into an individual plastic bag (e.g., "baggies"). Be sure to remove excess air from the bag and then secure tightly with a twist tie.

Shipping

  1. Enter the appropriate flock identification on the identification label and secure it to the master bag. Please use a permanent marker otherwise the ink will smudge. It is particularly important to include the growers name and the flock identification.

  2. Place into a Styrofoam shipping container with ice packs for next day delivery.

  3. Complete an Analysis Request form indicating the tests to be completed. Be sure to use a permanent marker.

  4. Enclose an Analysis Request form in a separate plastic bag to avoid smearing and place it in the shipping container. Send by overnight courier service or mail to:

Diversified Laboratories, Inc. 
4150 Lafayette Center Drive
Suite 600 
Chantilly, VA 20151 
Tel: 703-222-8700